Colloidal silver hiv virus

Crooks tells us there are no serious side effects whatsoever from high concentrations. Research scientist Dr. Gary Smith reports that he has noticed a correlation between low silver levels, sickness and immune deficiency. He found people who have low silver levels tend to be frequently sick and to have innumerable colds, flu's, fevers, and other illnesses. The research of Dr. Gary Smith would seem to support the belief that colloidal silver is an entirely natural healing agent.

First is the HIV virus, which attacks the immune system. Second are the main outward effects, the conditions resulting from the weakened immune system. Research evidence shows that Colloidal Silver is a two edged sword. It attacks the HIV virus directly and effectively, and then forms virtually its own immune system to ward off the various health problems the immune system has not been able to handle. Parasites are being recognised more and more as a cause of failing health.

Hulda Clark, Ph. Health Consciousness, Vol. However, tests have shown that mosquito larva are effectively killed by colloidal silver. It should be remembered that to rid oneself of parasites by killing the eggs or larvae means that one must continue the treatment until all of the adults die, probably of old age.

More research is needed. But if the AIDS or Cancer and other conditions can be effectively treated with colloidal silver, then any effects on parasites is a free benefit. In as much as AIDS is the result of a virtually destroyed immune system, it would be surprising to find that colloidal silver did not have a dramatic effect on AIDS.

AIDS is a viral disease, and antibiotics seldom have any effect on any virus, but the colloidal silver ion is highly effective. The evidence strongly supports the theory that colloidal silver is highly effective against all strains of virus, curing even the common cold in one dose.

Where many drugs on the market suppress certain symptoms of a cold, colloidal silver is said to kill off the virus that causes it to hang on. AIDS, like all other viral infections, is unaffected by antibiotics, which each kill off only a few strains of bacteria and none are effective against the virus, yeast's, or fungi. Yet, researchers are telling us that colloidal silver is highly effective against all of these. One serious condition typical of AIDS is rapid ageing. Ageing is generally considered as due to a slowing down of the body's ability to replace worn out cells fast enough.

This slowing down begins by the time of adulthood and continues into old age. From the research of Dr. Becker, it would seem colloidal silver will produce the dedifferentiated cells necessary to prevent this slowdown of cell replacement. It is probable that the weakened immune system is incapable of producing the undifferentiated cells necessary to rebuild worn out cells.

Colloidal silver produced the needed cells to make this possible. All of this is being discovered and rediscovered at the same time that disease bacterium are developing immunity to modern antibiotics.

Furthermore, immunity to the antibiotics seems to be developing all over the world, even in isolated areas. The medical profession is alarmed. Can silver save us? Many authorities think so! As single celled creatures called bacteria use a common type enzyme or 'chemical lung" for their oxygen metabolism, the presence of colloidal silver cripples the enzyme, therefore causing the organism to suffocate. Any and all bacteria are therefore killed within the 6-minute time frame, without causing any adverse effect on the surrounding tissue cells.

Dr Becker also states in his book: "Positive silver kills all types of bacteria". This is exiting, because no other single antibiotic works against all types of bacteria.

Positive silver, however, offers several advantages over previous forms. There are no ions besides silver to burden tide tissues. It works against all types of bacteria and viruses, even killing antibiotic resistant strains as well as all fungal infections.

To carry the fight to fungal infection, one must firstly understand fungal growths. A fungus is a series of single cells that have small tubes of the material from which the cell is made, which stretches between the cell walls. The data presented here contribute to a new and still largely unexplored area; the use of nanomaterials against specific targets of viral particles. Commercially manufactured nm silver nanoparticles, surface coated with 0. Stock solutions of silver nanoparticles, silver sulfadiazine Sigma-Aldrich and silver nitrate Sigma-Aldrich were prepared in RPMI cell culture media.

Following serial dilutions of the stock were made in culture media. They were kindly donated by Gadi Borkow. Aliquots of cell-free culture viral supernatants were used as viral inocula. Peripheral blood mononuclear cells PBMC were isolated from healthy donors using Histopaque Sigma-Aldrich according to the manufacturer's instructions. UC was kindly donated by Dr. Gadi Borkow. Then the cells were incubated for 48 h, and the amount of viral infection was quantified with the Beta-Glo Assay System Promega.

The amount of captured gp was detected by peroxidase-conjugated murine anti-gp MAb. Before the addition of the gp protein, plates were washed three times to remove unbound silver nanoparticles [ 27 ]. After incubation for 5 min and 60 min at room temperature, the mixtures were centrifuged three times at 10, rpm, the supernatant fluids removed, and the pellets washed three times.

The percentage of residual infectivity after silver nanoparticle treatment was calculated with respect to the positive control of untreated virus [ 31 ]. Time-of-addition experiment graphs are nonlinear regression curves done with SigmaPlot All authors read and approved the final manuscript. HHL participated in the conception and experimental design of the in vitro HIV-1 manipulation and infectivity assays, in analysis and interpretation of the data, and in writing and revision of this report.

National Center for Biotechnology Information , U. Journal List J Nanobiotechnology v. J Nanobiotechnology. Published online Jan Author information Article notes Copyright and License information Disclaimer.

Corresponding author. Humberto H Lara: moc. Received Jul 21; Accepted Jan This article has been cited by other articles in PMC. Abstract Background Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated.

Results Our data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Conclusions These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.

Range of antiviral activity Silver nanoparticles of nm were tested against a panel of HIV-1 isolates using indicator cells in which infection was quantified by a luciferase-based assay. Table 1 Antiviral effect of silver nanoparticles against HIV-1 strains. Open in a separate window. Antiviral activity of silver nanoparticles and ions To define that the observed antiviral effect of silver nanoparticles is due to nanoparticles, rather than just silver ions present in the solution, we also assessed the antiviral activity of silver sulfadiazine AgSD and silver nitrate AgNO 3 , known antimicrobial silver salts that exert their antimicrobial effect through silver ions [ 25 ].

Table 2 Antiviral effect of silver salts and nanoparticles against HIV Inhibition of viral adsorption To confirm that the anti-HIV activity of silver nanoparticles can be attributed to the inhibition of virus binding or fusion to the cells, a virus adsorption assay was performed [ 26 ].

Figure 1. Silver nanoparticles interfere with gpCD4 interaction The inhibitory activity of silver nanoparticles against the gpCD4 interaction was also investigated in a competitive gpcapture ELISA. Time Site of Intervention To further determine the antiviral target of silver nanoparticles, a time-of-addition experiment was performed using a single cycle infection assay. Figure 2. Virucidal activity of silver nanoparticles: inactivation of cell-free and cell-associated virus To study the effect that silver nanoparticles have over the virus itself, cell-free and cell-associated HIV-1 were treated with different concentrations of nanoparticles.

Figure 3. Figure 4. Discussion Silver nanoparticles proved to be an antiviral agent against HIV-1, but its mode of action was not fully elucidated. Conclusions Finally, we propose that the antiviral activity of silver nanoparticles results from their inhibition of the interaction between gp and the target cell membrane receptors. Methods Silver compounds Commercially manufactured nm silver nanoparticles, surface coated with 0.

Competing interests The authors declare that they have no competing interests. Authors' contributions All authors read and approved the final manuscript. Geneva, Switzerland. The incidence of multidrug and full class resistance in HIV-1 infected patients is decreasing over time in Portugal. Multi-targeting the entrance door to block HIV Curr Drug Targets Infect Disord. Totowa, NJ: Kinchington, D. F; Assessment of Activity of Topical Virucidal Agents; pp.

Interaction of silver nanoparticles with HIV Infect Dis Obstet Gynecol. Antimicrobial effect of surgical masks coated with nanoparticles. J Hosp Infect. Biological properties of "naked" metal nanoparticles. Adv Drug Deliv Rev. Antimicrobial effects of silver nanoparticles. Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res. The bactericidal effect of silver nanoparticles. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli.

Silver nanoparticles as antimicrobial agent: a case study on E. J Colloid Interface Sci. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chem Commun. Scientists studied the absorption spectra of the different preparations to pinpoint their shapes. Also, the UV-Visible spectra graphs helped the group determine nanoparticle sizes. Scientists tested, in vitro, each of three silver nanoparticle-preparations in HIV-1 cells.

Yacaman and his colleagues incubated the samples at 37 C. Plus, the foamy carbon was a slightly-better capping agent because of its free surface area. Size also played a role since none of the attached nanoparticles were greater than 10nm. Scientists think the nanoparticles bonded through the gp glycoprotein knobs on HIV-1, using the sulfur residues on the knobs.

Although this study shows silver nanoparticles may treat HIV-1, scientists need to research this relationship further. Scientists are forming a preventive cream for HIV-1, which they will test on humans. Scientists are also studying other uses for silver nanoparticles.

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